The trial was carried out during two consecutive years (2014 and 2015) in an experimental orchard belonging to the Andalusian Institute of Training and Agricultural Research (IFAPA, in Spanish); located in the Guadalquivir river basin, SW Spain (37º 301 47" N; 05º 581 0211 W) as the result of the established collaboration between IFAPA and Phytoplant Research S.L. with a climate is typically Mediterranean-dry with an annual rainfall of 550 mm and soil it is a Typical Fluvisol (USDA, 2016), with a useful depth up to 2.5 m with low content of organic matter

Three new hemp Phytoplant Research S.L varieties with CBD chemotype, registered in the European Union Community Plant Variety Office, (Sara, Pilar, Theresa) and two with CBG chemotype (Aida and Juani) were tested. All hemp varieties were grown under polyethylene-covered tunnel structures (plastic macro-tunnels), with an useful surface was 176 m2. These three varieties were transplanted on 28 May with a density for each high-tunnel of 9,777 plants· ha-1.

During the studied seasons, irrigation doses were estimated according to the methodology, and following the recommendations described in several previous and documented experiences.At the end of season, the total irrigation volume applied was close to 400 mm. Fertilization were performed using exclusively Terra Vega (CANNA España SL) and Terra Vega + Terra Flores,  (CANNA España SL). At the end of the season, plants had received 115-75-160 kg· ha-1 of NPK.

Throughout 2015, two new Phytoplant Research S.L varieties (Theresa and Juani with CBD and CBG chemotypes, respectively), were tested. All varieties were cultivated within the same high tunnels used in the previous season were transplanted at the end of May - beginning of June. During this second season, and considering the plant development in 2014, three different plant densities (PD) were tested 9,777 (PD1), 7,333 (PD2 )and 5,866 (PD3) plants ha-1.

Regarding the measurements, at the end of the studied seasons, fresh weight, dry weight was recorded, leaves and flowers were pulled apart from the stems, obtaining dry weight of leaves and flowers, and the dry weight of stems and branches separately. Additionally, the active biomass content in plant organs distributed in three equal positions was measured: upper, medium, and lower. Cannabinoids contents were measured by using the official method, (EC) No 1177/2000 of 31 May 2000 Annex C, named “Community method for the quantitative determination of THC content of hemp varieties”. The experimental design In 2014, was of randomized complete blocks, considering four replications per variety with an experimental plot with 40 plants. In 2015, the experimental design was the same as in the previous season, considering four replications per variety with 40, 32, and 24 plants per experimental plot,